Inclusion Body Hepatitis (IBH) has emerged as a primary disease in commercial broilers. Earlier it was identified to be associated with immunosuppressive agents like Infectious Bursal Disease virus and Chicken Infectious Anaemia virus (Gomis et al., 2006). Recent work has demonstrated virulent strains which alone can produce severe disease with mortality ranging from 10-30%.
Globion India has undertaken a study to investigate IBH affecting commercial broilers flocks in India. The study aims to isolate and characterize the FAdV types involved in the disease and thus provide actual data pertaining to the disease scenario
Abstract of the study
The occurrence of Inclusion Body Hepatitis in commercial broilers flocks of different parts of India was studied in order to identify the Fowl Adenovirus (FadV) type responsible for the disease. The most common gross lesions observed during necropsy included hydropericardium, hepatomegaly, petechial haemorrhages on liver, yellowish discolouration of subcutaneous and abdominal fat along with swelling and paleness of kidneys. Histopathological examination revealed intranuclear basophilic inclusion bodies indicative of FadV infection. Virus isolation was attempted in-vivo, in-ovo and in-vitro in Specific Pathogen Free (SPF) birds, SPF embryonated eggs and Chicken Embryo Liver primary cells, respectively. Antibody based detection of FAdV was suitably done with the help of Agar Gel Immunodiffusion test (AGID). Polymerase Chain Reaction and Restriction Fragment Length Polymorphism analysis was performed to detect and differentiate FadV types. Sequencing and phylogenetic analysis revealed presence of FadV type 2, 4, 8 and 11 in Indian poultry.
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